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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Lab Chip. 2019 Oct 9;19(20):3375–3386. doi: 10.1039/c9lc00506d

Figure 3. Detection of HIV virus amplified by dried RT-LAMP reagents.

Figure 3.

(A) There is no significant difference in test line intensity of labeled amplification products detected on LFIAs after amplification with fresh RT-LAMP reagents as with reagents dried for 21 days. n=5 (fresh), n=13 (dried), circles indicate replicates; *** indicates p ≤ 0.001 (B) Labeled RT-LAMP amplification products are visually detectable from as few as 1,000 HIV virus particles when reactions contain 16% serum. Electrophoresis gels verifying amplification (top, contrast increased for visualization), LFIA test results (middle), and LFIA test line quantification (bottom). n=3, circles indicate replicates; ** indicates p ≤ 0.01; * indicates p ≤ 0.05.