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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Lab Chip. 2019 Oct 9;19(20):3375–3386. doi: 10.1039/c9lc00506d

Figure 4. Detection of HIV virus from spiked blood on microRAAD with reagents dried for 21 days.

Figure 4.

(A) Representative μPADs imaged 90 minutes after blood with and without HIV virus deposited into microRAAD’s sample inlets. After capillary migration of HIV to RT-LAMP zone, the RT-LAMP zone and valves are automatically heated, releasing solution to LFIA for detection. As few as 105 HIV virus copies in (B) rehydrating mix alone or (C) rehydrating mix with 12μL of blood are detectable by microRAAD prepared with RT-LAMP reagents dried for 21 days. n=4 (B) and n=3 (C), replicates indicated by each circle; ** indicates p ≤ 0.05 and ** indicates p ≤ 0.01.