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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: J Funct Foods. 2017 Aug 10;37:310–321. doi: 10.1016/j.jff.2017.08.001

Fig. 5 -. TFS-induced apoptosis in A2780/CP70 and OVCAR-3 cells is related with p53 upregulation.

Fig. 5 -

(A-C) Expression levels of protein p53 and p-p53 in A2780/CP70 and OVCAR-3 cells were measured by western blot after treating with TFS (0, 0.5, 1.0 and 1.5 μg/ml) for 24 h. Each protein intensity was expressed as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 versus control. (D, E) Cellular apoptosis was measured after cells were treated with TFS and p53 inhibitor PFT-α for 24 h and then stained with Hoechst 33342. (F-K) Cell viability, caspase 3/7 and caspase 8 activities were determined after treatment with TFS and p53 inhibitor PFT-α for 24 h. *P < 0.05, **P < 0.01, and ***P < 0.001 versus control.