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. 2020 Jul 27;15(7):e0236481. doi: 10.1371/journal.pone.0236481

Fig 5. Expression levels of endogenous SlRDR6 and SlRDR1 mRNAs in PSTVd-infected transgenic ‘Moneymaker’ tomato plants.

Fig 5

Expression levels of endogenous SlRDR6 and SlRDR1 mRNAs were analyzed by RT-qPCR. The expression levels of SlRDR6 mRNA were significantly lower in PSTVd-infected SlRDR6i plants than in EC plants at 10 and 20 dpi except for 15 dpi. The expression levels of SlRDR1 mRNA were significantly higher in PSTVd-infected SlRDR6i plants than in EC plants at 15 dpi. The statistically significant difference between EC and SlRDR6i plants was confirmed by Welch’s or Student’s t-test. The relative expression levels of endogenous SlRDR6 and SlRDR1 mRNAs were calculated for each time point with the value of the mock-inoculated EC plants as a standard. qPCR was performed with the PCR primer for endogenous SlRDR6 mRNA, or endogenous SlRDR1 mRNA. Mean values are based on three biological replicates of the pooled sample which is collected from five individual plants.