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Accumulation of PSTVd-sRNA was analyzed by Northern-blot hybridization with DIG-labeled cRNA probe for PSTVd. Loadings were equalized by the signal intensity of rRNA and tRNA stained with ethidium bromide. PSTVd-sRNA derived from PSTVd-Int or PSTVd-RG1 genomic RNA was highly accumulated in EC plants compared with that in SlRDR6i plants.
