. 2020 Jul 27;15(7):e0232874. doi: 10.1371/journal.pone.0232874

Table 1. Nested PCR protocol and Plasmodium ssrRNA genes used in this study.

Target species	Primer	Sequence (5'-3')	Reaction
Plasmodium genus-specific	rPLU1	`TCAAAGATTAAGCCATGCAAGTGA`	Nested 1
	rPLU5	`CCTGTTGTTGCCTTAAACTTC`
	rPLU3	`TTTTTATAAGGATAACTACGGAAAAGCTGT`	Nested 2
	rPLU4	`TACCCGTCATAGCCATGTTAGGCCAATACC`
Plasmodium species-specific
Plasmodium falciparum	rFAL1	`TTAAACTGGTTTGGGAAAACCAAATATATT`	Nested 2
	rFAL2	`ACACAATGAACTCAATCATGACTACCCGTC`
Plasmodium malariae	rMAL1	`ATAACATAGTTGTACGTTAAGAATAACCGC`	Nested 2
	rMAL2	`AAAATTCCCATGCATAAAAAATTATACAAA`
Plasmodium ovale	rOVA1	`ATCTCTTTTGCTATTTTTTAGTATTGGAGA`	Nested 2
	rOVA2	`GGAAAAGGACACATTAATTGTATCCTAGTG`