Table 1. Chlamydia trachomatis DNA recovery from spiked surfaces.
Surface | Spiked concentration (omcB copies/μL) | omcB loge load range | Mean loge omcB load (sd) | Mean percentage recoverya |
---|---|---|---|---|
Spiked swab | 1000 | 7.14–7.72 | 7.43 (0.22) | 100% |
100 | 5.22–5.76 | 5.57 (0.18) | 100% | |
10 | 3.68–3.99 | 3.87 (0.15) | 100% | |
1 | 1.73–2.05 | 1.99 (0.13) | 100% | |
Plastic | 1000 | 6.92–7.40 | 7.17 (0.21) | 97% |
100 | 5.15–5.47 | 5.26 (0.13) | 95% | |
10 | 3.24–3.64 | 3.85 (0.13) | 91% | |
1 | 1.34–2.05 | 1.84 (0.27) | 92% | |
Skin | 1000 | 5.06–5.38 | 5.22 (0.18) | 70% |
100 | 3.77–4.06 | 3.92 (0.16) | 70% | |
10 | 1.80–2.25 | 2.12 (0.17) | 55% | |
1 | 1.01–1.25 | 1.17 (0.09) | 59% | |
Cotton cloth | 1000 | 3.95–4.55 | 4.29 (0.28) | 58% |
100 | 2.05–2.53 | 2.25 (0.18) | 41% | |
10 | 0.90–1.15 | 1.06 (0.11) | 27% | |
1 | 0.00–0.86 | 0.14 (0.35) | 7% | |
Woven mat | 1000 | 0.00–0.00 | 0.00 (0.00) | 0% |
100 | 0.00–0.00 | 0.00 (0.00) | 0% | |
10 | 0.00–0.00 | 0.00 (0.00) | 0% | |
1 | 0.00–0.00 | 0.00 (0.00) | 0% |
aMean percentage recovery compared to loge omcB load detected on spiked swabs.