Fig 6. Experimental testing of model predictions using single and pairwise drug combinations.

Several single and pairwise perturbations that were predicted to have a differing phenotypic effects were experimentally tested in the melanoma cell line A2058 (highlighted in Fig 5). Cells were perturbed with drugs that target nodes in the computational model (top). During perturbation, cells were subjected to live-cell imaging, the resulting images were segmented, and cell count was quantified, normalized relative to no-drug control and log₂-transformed (see growth curves in middle panel). Agreement between model prediction (log₂-transformed, normalized and averaged growth) and experiment is summarized (bottom panel).