CapZyme-Seq: A 5′-RNA-Seq Method for Differential Detection and Quantitation of NAD-Capped and Uncapped 5′-Triphosphate RNA

. 2020 Jun 3;1(1):100002. doi: 10.1016/j.xpro.2019.100002

Reagents	Assay with NTPs and NAD⁺ Volume, μL	Assay with NTPs Only Volume, μL
ATP, 5 mM	2	2
CTP, 5 mM	2	2
GTP, 5 mM	2	2
UTP, 5 mM	2	2
NAD⁺, 100 mM	2	-
Heparin, 10 μg/μL	1	1
500 mM Tris-HCl pH 8.0	2	2
H₂O	7	9
Total:	20	20