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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Anal Chem. 2020 Jun 16;92(13):9146–9155. doi: 10.1021/acs.analchem.0c01449

Figure 4.

Figure 4.

(a) HCD and (b) 193 nm UVPD (8 pulses, 2.5 mJ per pulse) mass spectra of m/z 704.49 from Proteus mirabilis lipid extract. (c) Fragment ion map of m/z 704.49 identified as PE 18:1(11Δ) _14:0 OH with the hydroxyl moiety highlighted in green. (Fragmentation sites are color-coded to correspond to the ions identified in panels a and b.) (d) Doughnut plot displaying the frequency of the occurrence of hydroxyl GPL acyl chains by sample. For example, acyl chain 16:1 OH was the most common unsaturated acyl chain because it was identified in 8 of the 12 investigated bacterial extract samples, whereas acyl chain 18:0 OH was identified in only 1 of the 12 bacterial extracts.