Fig 4. Active labor is associated with recruitment of RNAPII to labor-driving genes and eRNA expression at gene-adjacent intergenic regions with H3K27ac peaks.

(A) Metagene plot exhibiting log₂-fold RNAPII signal, normalized to input, at bodies of genes whose intron read–based expression levels are enriched in d19 LAB samples relative to d15 samples. Data associated with this figure can be found in S6 Data. (B) Violin plots displaying log₂-fold RNAPII signal (RPM), normalized to input, at promoters of genes whose intron read–based expression levels are enriched in d19 LAB samples relative to d15 samples. Groups labeled with different letters show significant difference, with p < 0.05. Data associated with this figure can be found in S10 Data. (C) RNA-seq, RNAPII, and H3K27ac ChIP-seq reads (RPM) mapped at the Fos locus in d15 or d19 LAB samples. Regions containing RNAPII (red bars) or H3K27ac peaks (blue bars) indicated in d15 and d19 LAB samples. RNA-seq profile at labor–up-regulated eRNA-exhibiting region (situated between the dashed lines) downstream of Fos is presented at a different scale compared with the rest of the locus. (D) eRNA volcano plot highlighting intergenic H3K27ac peaks in genomic regions that exhibit significant differences in eRNA levels between d15 and d19 LAB samples. (E) All enriched transcription factor motifs identified at genomic regions that feature intergenic H3K27ac peaks and exhibit a significant increase in eRNA levels in d19 LAB samples compared with d15 samples. (F) The top 10 enriched motifs identified at promoters for genes that exhibit a significant increase in intronic RNA levels for d19 laboring samples compared with d15 samples. ChIP-seq, chromatin immunoprecipitation with massively parallel sequencing; chr12, chromosome 12; d, day; eRNA, enhancer RNA; FC, fold-change; Fos, FBJ osteosarcoma oncogene; H3K27ac, H3 acetylation on lysine residue 27; LAB, active labor; NS, nonsignificant; RNAPII, RNA polymerase II; RNA-seq, RNA-sequencing; RPM, reads per million; P(adj), adjusted p-value; TES, transcription end site; TSS, transcription start site.