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. 2020 Jul 6;57(3):791–803. doi: 10.3892/ijo.2020.5094

Figure 4.

Figure 4

Effect of SLRP downregulation on chondrosarcoma cell growth. (A) Cells were harvested and seeded (5,000 cells/well) in 96-well plates. HTB94 cells were transfected with siRNAs specific for lumican, decorin and biglycan, for 48 h. A non-specific RNA sequence was used as a control (siScr). Cell number was determined using a fluorometric CyQUANT Assay kit. (B) Cells were harvested and seeded (12,000 cells/well) in 96-well plates. Following incubation with siRNAs specific for lumican, decorin and biglycan for 48 h, cell number was determined using a Vybrant MTT Assay kit. Results represent the average of 3 separate experiments performed in triplicate. Data are the means ± SEM; ***P≤0.001, statistically significant difference compared with the respective control samples. NS, not significant; SLRPs, small leucine-rich proteoglycans.