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. 2020 Jul 6;57(3):791–803. doi: 10.3892/ijo.2020.5094

Figure 5.

Figure 5

Effect of SLRP downregulation on chondrosarcoma cell migration and adhesion. (A) Cells were harvested and seeded (100,000 cells/well) in 24-well plates, to be treated with the siRNAs specific for 3 SLRPs, lumican, decorin and biglycan for 48 h (at which point they were confluent). Following treatment, the cell layer was 'scratched' with a 10 µl sterile pipette tip, and the wound surface area was measured at the 0, 6 and 12 h points. (B) The cell substratum surface area was measured utilizing the ImageJ program and expressed as arbitrary units. (C) HTB94 cells were treated with siRNAs specific for 3 SLRPs, lumican, decorin and biglycan for 48 h. A non-specific RNA sequence was used as a control (siScr). Following treatment, cells were harvested and seeded (10,000 cells/well) for 1 h at 37°C in 96-well plates coated with fibronectin. The number of attached cells was determined using fluorometric CyQUANT Assay kit Results represent the average of 3 separate experiments. Data are the means ± SEM. NS, not significant; SLRPs, small leucine-rich proteoglycans.