Table II.
Primer sequences used for quantitation of MMPs and TIMPs.
| A, MMPs.
| ||
|---|---|---|
| Gene | Direction | Sequences |
| MMP2 | Forward | 5′-AACTACGATGACGACCGCAAGT-3′ |
| Reverse | 5′-AGGTGTAAATGGGTGCCATCA-3′ | |
| MMP3 | Forward | 5′-TTCCGCCTGTCTCAAGATGATAT-3′ |
| Reverse | 5′-AAAGGACAAAGCAGGATCACAGTT-3′ | |
| MMP7 | Forward | 5′-CTTTGCGCGAGGAGCTCA-3′ |
| Reverse | 5′-CAGGCGCAAAGGCATGA-3′ | |
| MMP9 | Forward | 5′-AGGCGCTCATGTACCCTATGTAC-3′ |
| Reverse | 5′-GCCGTGGCTCAGGTTCA-3′ | |
| MMP10 | Forward | 5′-GGACCTGGGCTTTATGGAGATAT-3′ |
| Reverse | 5′-CCCAGGGAGTGGCCAAGT-3′ | |
| MMP11 | Forward | 5′-GGGTGCCCTCTGAGTCGA-3′ |
| Reverse | 5′-TCACAGGGTCAAACTTCCAGTAGA-3′ | |
| MMP13 | Forward | 5′-AAATTATGGAGGAGATGCCCATT-3′ |
| Reverse | 5′-TCCTTGGAGTGGTCAAGACCTAA-3′ | |
|
| ||
| B, TIMPs. | ||
|
| ||
| Gene | Direction | Sequences |
|
| ||
| TIMP1 | Forward | 5′-GACGGCCTTCTGCAATTCC-3′ |
| Reverse | 5′-GTATAAGGTGGTCTGGTTGACTTCTG-3′ | |
| TIMP2 | Forward | 5′-GAGCCTGAACCACAGGTACCA-3′ |
| Reverse | 5′-AGGAGATGTAGCACGGGATCA-3′ | |
| TIMP3 | Forward | 5′-CCAGGACGCCTTCTGCAA-3′ |
| Reverse | 5′-CCCCTCCTTTACCAGCTTCTTC-3′ | |
| TIMP4 | Forward | 5′-CACCCTCAGCAGCACATCTG-3′ |
| Reverse | 5′-GGCCGGAACTACCTTCTCACT-3′ | |
MMP, matrix metalloproteinase; TIMP, tissue inhibitors of metalloproteinase.