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. 2020 Jun 23;9:e53889. doi: 10.7554/eLife.53889

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© 2020, Zavortink et al

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Figure 6. — (A) The interaction between ME31B-GFP and RanBPM does not depend on Kdo. ME31B-GFP complexes were immunoprecipitated from 1–2 hr embryo lysates using α-GFP or IgG (as a control). Immunoprecipitations were performed in 1–2 hr embryo lysates with Kdo knocked down. *, non-specific band. (B) The interaction between ME31B-GFP and RanBPM does not depend on PNG. As in B, except in 1–2 hr png⁵⁰ embryo lysates. *, non-specific band. (C) The interaction between Cup and RanBPM does not depend on PNG. Complexes were immunoprecipitated from 1–2 hr lysates from wild-type (w1118) or png⁵⁰ embryos using α-Cup or IgG. Membranes were probed with α-Cup or α-RanBPM. *, non-specific band.