Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | c-Myc | GeneBank | ||
| strain, strain background (Escherichia coli) | DH5a | NEB | Catalog #C2987I |
High efficiency competent cells |
| strain, strain background (Escherichia coli) |
XL-10-Gold | Agilent | Catalog #200314 | Ultracompetent Cells |
| genetic reagent (include species here) | ||||
| cell line (Homo sapiens) | U-2 OS |
ATCC | ATCC HTB-96 |
Lack glucorticoid response |
| cell line (Homo sapiens) |
Lentivirus-expressed MYC, mCherry-reporter double-stable cell lines in U-2OS |
This paper |
bone osteosarcoma |
Cells maintained in D.Levens lab |
| cell line (Rattus norvegicus (Rat)) |
Myc-ER HO15.19 |
RRID:CVCL_0311 O'Connell et al., 2003 | ||
| transfected construct |
Palmitoyl-turquoise2 | gift from Dorus Gadella; | Addgene plasmid # 36209 |
http://n2t.net/addgene
Goedhart et al., 2012 |
| transfected construct |
Pd4EGFP-c-Myc and Myc mutants |
This Paper | ||
| transfected construct |
PTripZ-EGFP-cMyc and mutant |
This paper |
||
| transfected construct |
PLVX-PKG-GAL4USA-(Ebox)-E1bTATA-mcherry-hygro (Plasmid) |
This Paper |
||
| biological sample (include species here) | ||||
| antibody | anti-cMyc (rabbit monoclonal) |
Abcam | Catalog #ab-32072 |
1:5000 |
| antibody | anti-GFP rabbit polyclonal |
Abcam | Catalog #ab-32146 |
1:1000 |
| antibody | anti-GAPDH | Abcam | Catalog #ab-9485 | 1:2500 |
| recombinant DNA reagent | Pd4EGFP |
This paper |
||
| recombinant DNA reagent | PTripZ-EGFP |
This paper |
||
| recombinant DNA reagent | pLVX-mCherry-N1 |
Takara | Catalog #632562 | |
| sequence-based reagent |
PKG-F | This Paper |
CR primer |
GTGAGCGGCCGCGACTCTGAGTAATTCTACCGGGTAGG
|
| sequence-based reagent |
PKG-R |
This Paper |
PCR primer |
GTGAGCGGCCGCGACTCTGAGTAATTCTACCGGGTAGG
|
| sequence-based reagent |
Hygro1-F | This Paper |
PCR primer |
GCCCAAGCTTACCATGAAAAAGCCTGAACTCACC |
| sequence-based reagent |
Hygro1-R | This Paper | PCR primer |
TGTTGGAGCCGAAATCCGCGTGCA
|
| sequence-based reagent |
Hygro2-F |
This Paper |
PCR primer |
GGATTTCGGCTCCAACAATGTCCTGACGGACAATG
|
| sequence-based reagent |
Hygro2-R | This Paper | PCR primer | GGATTTCGGCTCCAACAATGTCCTGACGGACAATG |
| peptide, recombinant protein | ||||
| commercial assay or kit |
Direct-zol RNA miniprep kit |
Zymo Research |
Catalog #R2051 | |
| commercial assay or kit |
Luna Cell Ready One-Step RT-qPCR Kit |
NEB |
Catalog #E3031, #E3005, #E3006 |
|
| commercial assay or kit |
Lipofectamine 2000,3000 transfection kit |
Thermo-Fish (Invitrogen) |
Catalog #L2000, L3000-008 |
|
| commercial assay or kit |
Lenti-X Accelerator kit |
Takara |
Catalog #631257 |
|
| commercial assay or kit |
Lenti-X Packaging Single Shot kit |
Takara | Catalog #631275 |
|
| commercial assay or kit |
the ChIP-IT High Sensitivity kit | Active Motif | Catalog #53040 |
|
| chemical compound, drug |
Dexamethasone (Dex) |
Sigma | ||
| chemical compound, drug |
Promegestone |
PerkinElmer Life Sciences |
R5020 |
|
| chemical compound, drug |
10058-F |
Sigma | Catalog #F3680 |
|
| chemical compound, drug |
4-OH tamoxifen |
Sigma-Aldrich |
Catalog #94873 |
|
| chemical compound, drug |
All the restriction enzymes and DNA polymerase |
NEB | ||
| software, algorithm |
KaleidaGraph | Synergy Software, Reading, PA |
||
| software, algorithm |
NIS-Element |
Nikon | ||
| software, algorithm |
MATLAB | Mathworks | ||
| software, algorithm |
LightCycler 480 Probes master |
Roche-applied Science | ref number 04707494001 | |
| other |