Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) |
TLN2 | Human Genome Nomenclature Database | HGNC:15447 | |
| Gene (Homo sapiens) |
VCL | Human Genome Nomenclature Database | HGNC:12665 | |
| Strain, strain background (Escherichia coli) | BL21-Gold (DE3) | Agilant | Cat# 230132 | |
| Strain, strain background (Escherichia coli) | XL1-Blue | Agilant | Cat# 200249 | |
| Recombinant DNA reagent | pCB-a-bax33-hsTln2-3C-SNAP-6His | This paper, Subcloned from gift from Carsten Grashoff |
includes L435G mutation to reduce calpain cleavage | |
| Recombinant DNA reagent | pCB-a-bax33-hsTln2ABS2-3C-SNAP-6His | This paper | L435G, K925E, Q926E, Q933E, R1502E, R1512E, K1524E | |
| Recombinant DNA reagent | pCB-a-bax33-hsTln2ABS3-3C-SNAP-6His | This paper | L435G, K2244D, K2245D, K2246D | |
| Recombinant DNA reagent | pCB-a-bax33-hsTln2ABS2/3-3C-SNAP-6His | This paper | L435G, K925E, Q926E, Q933E, R1502E, R1512E, K1524E, K2244D, K2245D, K2246D | |
| Recombinant DNA reagent | pCB-a-bax33-hsTln2N:R3-3C-SNAP-6His | This paper | Tln2(1-910) with L435G mutation | |
| Recombinant DNA reagent | pCB-a-bax33-hsVcl-3C-SNAP-6His | This paper, Subcloned from gift from Carsten Grashoff |
||
| Recombinant DNA reagent | pCB-a-bax33-hsVcl2A-3C-SNAP-6His | This paper | N773A,E775A | |
| Recombinant DNA reagent | pCB-a-bax33-hsVclD1-3C-SNAP-6His | This paper | Vcl(1-258) | |
| Rcombinant DNA reagent | pCB-a-bax33-hsVclHead-3C-SNAP-6His | This paper | Vcl(1-823) | |
| Recombinant DNA reagent | pCB-a-bax33-hsVclTali-3C-SNAP-6His | This paper | Vcl(878–1066) | |
| Recombinant DNA reagent | pCB-a-bax33-hsVclTail3Q-3C-SNAP-6His | This paper | Vcl(878–1066) K944Q, R944Q, K1061Q | |
| Recombinant DNA reagent | pCB-a-bax33-hsVcl3Q-3C-SNAP-6His | This paper | K944Q, R945Q, K1061Q | |
| Peptide, recombinant protein | 3C-6his protease | This lab | Purified from E. coli- BL21-Gold (DE3) | |
| Peptide, recombinant protein | Actin Rabbit skeletal muscle alpha actin |
Hypermol | Cat# 8101–01 | |
| Peptide, recombinant protein | Actin-ATTO488 Rabbit skeletal muscle alpha actin |
Hypermol | Cat# 8153–02 | |
| Peptide, recombinant protein | Actin-ATTO488-TIRF Rabbit skeletal muscle alpha actin |
Hypermol | Cat# 8153–03 | |
| Peptide, recombinant protein | Actin-biotin Rabbit skeletal muscle alpha actin |
Hypermol | Cat# 8109–01 | |
| Peptide, recombinant protein | VBS (vinculin activating peptide) | Hypermol | Cat# 8317–01 | Derived from C-terminal talin sequence |
| Peptide, recombinant protein | NeutrAvidin | Thermo Fischer Scientific | 31000 | |
| Chemical compound, drug | mPEG-silane, MW 2000 | Laysan Bio Inc | Cat# MPEG-SIL-2000–1 g | |
| Chemical compound, drug | mPEG-silane-biotin. MW 3400 | Laysan Bio Inc | Cat# Biotin-PEG-SIL-3400–1 g | |
| Chemical compound, drug | Methyl Cellulose | Acros Organics | Cat# 10287200 | viscosity 4000 c |
| Peptide, recombinant protein | Glucose Oxidase Type II | Sigma Aldrich Chemie GmbH | G6125 | From aspergillus |
| Peptide, recombinant protein | Catalase | Sigma Aldrich Chemie GmbH | E3289 | From bovine liver |
| Software, algorithm | FIJI | https://imagej.net/Fiji/Downloads | ||
| Software, algorithm | Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ | |
| Other | DOPC (18:1 (Δ9-Cis) PC) | Avanti Polar Lipids | Cat# 850375 | 1,2-dioleoyl-sn-glycero-3-phosphocholine |
| Other | DOPE (18:1 (Δ9-Cis) PE) |
Avanti Polar Lipids | Cat# 850725 | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine |
| Other | DOPS (18:1 PS) |
Avanti Polar Lipids | Cat# 840035 | 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) |
| Other | TopFluor TMR PC | Avanti Polar Lipids | Cat# 810180 | 1-oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-2-propionyl)amino)hexanoyl)-sn-glycero-3-phosphocholine |
| Other | Brain PI(4,5)P2 | Avanti Polar Lipids | Cat# 840046 | L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) (ammonium salt) |
| Other | 18:0-20:4 PI(4,5)P2
synthetic |
Avanti Polar Lipids | Cat# 850165 | 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-myo-inositol-4',5'-bisphosphate) |
| Other | sticky-Slide VI 0.4 | Ibidi | Cat# 80608 |