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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Cell Rep. 2020 Feb 25;30(8):2776–2790.e6. doi: 10.1016/j.celrep.2020.01.093

Figure 3. TIFAB Regulates USP15-Dependent Signaling in Hematopoietic Cells.

Figure 3.

(A) Schematic overview of TIFAB and USP15 substrates exerting homeostatic control of p53, left, and de-repression of p53 by deletion of TIFAB (middle) or by deletion of USP15 (right).

(B) Immunoblotting of HA-K48-Ub on lysates immunoprecipitated for MDM2 that were isolated from HEK293T cells transfected with TIFAB, USP15, and/or MDM2. Transfected cells were incubated with the proteasome inhibitor (MG-132) to enrich for ubiquitinated MDM2.

(C) Immunoblotting of Usp15, Keap1, and vinculin in Tifab+/+ and Tifab−/− BM mononuclear cells.

(D) Immunoblotting of Mdm2, p53, and vinculin in Tifab+/+ and Tifab−/− Lin− BM cells.

(E) Immunoblotting of USP15, Keap1, p53, and vinculin in Usp15+/+ and Usp15−/− BM mononuclear cells.

(F) Immunoblotting of Mdm2 and vinculin in Usp15+/+ and Usp15−/− BM mononuclear cells.