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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Invest Dermatol. 2020 Jan 28;140(8):1629–1638. doi: 10.1016/j.jid.2019.12.030

Figure 3. Effects of overexpression of GAS5 on induction of the pro-inflammatory macrophage phenotype, and expression of M1 macrophage marker genes.

Figure 3.

(a) Overexpression of GAS5 mRNA was achieved by plasmid transfection and confirmed by RT-qPCR (mean±SD, n=3 per group). GAS5 gene expression was assessed in RAW macrophages transfected with pGAS5 or transfected with pcDNA3.1. (b) Expression of M1 marker genes iNOS, IL1-beta, and TNFa following transfection of RAW macrophages with pGAS5 (mean±SD, n=5 per group). (c) Expression of M2 marker genes Arg1 and Mrc1 following transfection of RAW macrophages with pGAS5 (mean±SD, n=5 per group). (d) Flow cytometric analysis of M1 macrophage surface markers CD11b and CD86 (28.4%+2.03% vs 3.15%+1.08%, n=3, p < 0.001) in RAW cells overexpressing GAS5.