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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Invest Dermatol. 2020 Jan 28;140(8):1629–1638. doi: 10.1016/j.jid.2019.12.030

Figure 4. GAS5 induces STAT1 expression in macrophages.

Figure 4.

(a) STAT1, STAT3, and STAT6 gene expression in RAW macrophages transfected with pGAS5 or pcDNA3.1 (n=3, p < 0.05). (b) GAS5 and STAT1 gene expression in macrophages transfected with increasing amounts of pGAS5. (c) Western blot analysis of pSTAT1, STAT1, pSTAT3, STAT3, pERK, and ARG1 following transfection of RAW macrophages with pGAS5. GAPDH served as an internal loading control. (d) Quantification of relative intensities for the ratio of pSTAT1/STAT1, ARG1/GAPDH, and pERK/GAPDH from western blot.