Table 1.
Primers | Sequences (5’–3’) | |
Beta-actin | F | TCCCTGGAGAAGAGCTACG |
R | GTAGTTTCGTGGATGCCACA | |
ALDH | F | AGATGTGGACAAGGCAGTGAA |
R | ATCCACCAGGTAGGAGATGAC | |
KLF4 | F | ACCTTCTTCACCCCTAGAGC |
R | CCCAGTCACAGTGGTAAGGT | |
HMGA2 | F | TGGGAGGAGCGAAATCTAAA |
R | TCCCTGGAGAAGAGCTACG | |
c-Myc | F | CACATCAGCACAACTACGCA |
R | GCTCCAAGACGTTGTGTGT | |
Nanog | F | CAATGGTGTGACGCAGGGAT |
R | TGCACCAGGTCTGAGTGTTC | |
CD44 | F | CAAGCCACTCCAGGACAAGG |
R | ATCCAAGTGAGGGACTACAACAG | |
Kras | F | CGTAGGCAAGATGCCTTGA |
R | CCTCTTGACCTGCTGTGTCG | |
miRNA | Target Sequence | |
miR-181a | AACAUUCAACGCUGUCGGUGAGU | |
miR-181b | AACAUUCAUUGCUGUCGGUGGGU | |
Let-7 | UGAGGUAGUAGGUUGUAUAGUU | |
miR-21 | UAGCUUAUCAGACUGAUGUUGA | |
miR-155 | UUAAUGCUAAUCGUGAUAGGGGUU | |
miR-200c | CGUCUUACCCAGCAGUGUUUGG | |
miR-34c | AGGCAGUGUAGUUAGCUGAUUGC |