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. Author manuscript; available in PMC: 2021 Mar 16.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2020 Mar 16;84:185–193. doi: 10.1101/sqb.2019.84.039750

Figure 2.

Figure 2.

(A) A cartoon illustrating that dmDcr-2 uses its Platform•PAZ domains to recognize substrates with 3′ overhangs (self) and the helicase domain to recognize dsRNA with blunt termini (non-self). (Bottom) Color coding of domains, used throughout the figure. (B) Superimposition of dmDcr-2 helicase conformations shows an open, C-shaped, conformation in the apo structure (light, PDB: 6BUA) and a closed conformation in the presence of blunt dsRNA and an ATP analog (dark, PDB: 6BU9). In the closed conformation, Hel2 and Hel2i swivel toward Hel1 to clamp on the dsRNA terminus. (C) Structure of the hsDcr•TRBP•pre-let-7 complex with domains labeled (PDB: 5ZAL). Only the carboxy-terminal Type B motif of TRBP was resolved in the structure (Liu et al. 2018).