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. Author manuscript; available in PMC: 2021 Jul 23.
Published in final edited form as: Cell. 2020 Jun 30;182(2):404–416.e14. doi: 10.1016/j.cell.2020.06.006

Figure 5. Glutamine starvation induces ribosome collision-mediated ZAKα activation.

Figure 5.

(A) p38 phosphorylation in HeLa cells grown in rich or glutamine-depleted media pretreated with vehicle (DMSO) or ISRIB compared to stimulation by intermediate-dose ANS (1 mg/L, 15min). (B) p38 phosphorylation in WT or ZAK KO MCF10A cells in media with or without glutamine. (C) Immunoblots of a Phos-tag gel for ZAKα phosphorylation in ZAKα-FLAG HeLa cells grown in media containing 2, 0.02 or 0 mM glutamine pretreated with DMSO or ISRIB. (D) eIF2α phosphorylation induced by glutamine starvation in WT or ZAK KO MCF10A cells pretreated with DMSO or ISRIB.