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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Respir Physiol Neurobiol. 2020 Jun 3;279:103471. doi: 10.1016/j.resp.2020.103471

Figure 3: 5-HT2B receptor expression in the putative phrenic motor nucleus and non-phrenic ventral horn in pre-symptomatic wild-type and SOD1G93A mutant in C4 spinal cord sections.

Figure 3:

The representative photomicrographs display motor neurons (NeuN; red) and 5-HT2B (green) receptor expression within the putative phrenic motor nucleus (yellow dashed circle) and the non-phrenic ventral horn (area outside of the yellow dashed circle) from rat C4 spinal cord sections in pre-symptomatic (PS; 3A-J) wild-type and SOD1G93A (Mutant; MT; 3F-J) rats at 4x (3A&F), 20x (3B,D,G,I), and 40x (3C,E,H,J) magnification. The white arrows in the 20x magnification images indicate what neurons are displayed in the 40x magnification images. 5-HT2B receptor expression is represented as optical density (AU; 3K) and fractional area (3L) in the putative phrenic motor nucleus and non-phrenic ventral horn. 5-HT2B optical density and fractional area was not different in MT PS rats within the putative phrenic motor nucleus as well as within the non-phrenic ventral horn when comparing MT rats to age-matched WT littermates (p>0.05).