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. 2020 Jul 21;11:1405. doi: 10.3389/fimmu.2020.01405

Figure 1.

Figure 1

Accumulation of IPP and DMAPP in target cells after treatment with PTA. (A) The mevalonate pathway and the metabolites in the upstream of FDPS. Isoprenoid metabolites are synthesized from acetyl- CoA via mevalonate through IPP and DMAPP. By the action of isomerase, IPP is converted into DMAPP. FDPS, a target of PTA, catalyzes the synthesis of GPP from IPP and DMAPP, and that of FPP from GPP and IPP. Metabolites in the direct upstream of FDPS are IPP and DMAPP. (B) Mass spectrometric analysis of IPP and DMAPP in target cells after treatment with PTA. After treatment of Raji Burkitt's lymphoma cells with PTA (0, 50, 100 nM) for 2 h, the samples were analyzed through LC-MS and the extracted ion chromatograms (XIC) for m/z = 244.9985 were depicted. (C) Identification of IPP and DMAPP using standard compounds. The retention time for the standard IPP was 29.9 min and that for standard DMAPP was 30.3 min.