FIGURE 2.

Alterations NSC activation in DS mice. (A) Representative confocal-microscopy images of the DG of WT (left) and DS (right) mice after staining for GFAP (NSCs and astrocytes), S100β (astrocytes), and the proliferation marker Ki67. DAPI was used to stain cell nuclei. Ki67⁺-cells were restricted to the SGZ in WT and DS mice (B). Fewer proliferating cells (Ki67⁺) were found in the DS mice (C). (D) High-magnification confocal-microscopy images of dividing NSCs in WT (left) and DS (right, note the ramified morphology). The total number (E) as well as the proportion of dividing (Ki67⁺) NSCs was lower in the DS mice (F). *p < 0.05 by Student’s t-test. n = 5 for each condition. Bars show mean ± SEM. Dots show individual data. Scale bar in (A) is 40 μm, 50 μm in (B) and 5 μm in (D).