Table 1.
Antibody cocktails for immunophenotyping PBMCs by mass cytometry.
| Marker | mAb | Isotope label | |
|---|---|---|---|
| a) Surface stain 1 | CD45* | HI30 | Pd_104 |
| CD45* | HI30 | Pd_108 | |
| CCR2 | K036C2 | Eu_151 | |
| CD183 (CXCR3) | REA232 | Dy_163 | |
| CD184 (CXCR4) | 12G5 | Lu_175 | |
| CCR7 | G043H7 | Tb_159 | |
| b) Surface stain 2 | IgD | IA6-2 | Y_89 |
| CD11c | Bu15 | In_115 | |
| CD19 | HIB19 | Nd_142 | |
| CD56 | NCAM16.2 | Nd_143 | |
| CD4 | RPA-T4 | Nd_145 | |
| CD8a | RPA-T8 | Nd_146 | |
| CD20 | 2H7 | Sm_147 | |
| CD16 | 3G8 | Nd_148 | |
| CD25 | M-A251 | Sm_149 | |
| CD275 (ICOSL) | MIH12 | Nd_150 | |
| CD45RO | UCHL1 | Gd_152 | |
| CD68 | Y1/82A | Eu_153 | |
| CD31 | WM59 | Gd_155 | |
| CD86 | IT2.2 | Gd_156 | |
| CD123 | 6H6 | Dy_161 | |
| CCR6 | 11A9 | Pr_141 | |
| CX3CR1 | 2A9-1 | Er_164 | |
| CD61 | VI-PL2 | Ho_165 | |
| CD34 | 581 | Er_166 | |
| CD27 | M-T271 | Er_167 | |
| CD45RA | HI100 | Tm_169 | |
| CD3 | UCHT1 | Er_170 | |
| CD38 | HIT2 | Yb_172 | |
| CD14 | M5E2 | Yb_173 | |
| HLA-DR | G46-6 | Yb_174 | |
| CCR5 | HEK/1/85a | Nd_144 | |
| CD127 | A019D5 | Lu_176 | |
| CD11b | ICRF44 | Bi_209 | |
| CCR4 | L291H4 | Gd_158 | |
| c) Intra-cellular stain | IDO-1 | 700838 | Gd_154 |
| T-bet | 4B10 | Gd_160 | |
| FoxP3 | PCH101 | Er_162 | |
| Ki67 | B56 | Er_168 | |
| Arginase I | 14D2C43 | Yb_171 |
*
CD45 is on two isotopes in order to barcode patients (Pd_104) and controls (Pd_108). CD45 stains all leukocytes. mAb, monoclonal antibody.