Fig. 1. The CTD of Rpf2 is essential for cell growth at 16 °C.

a Left: growth of yeast strains containing truncated or mutated Rpf2 protein on solid medium. Serial dilutions (1 : 10 to 1 : 10,000) were spotted onto selective solid medium containing either galactose or glucose and incubated at 30 °C and 16 °C. Right: schematic representation of truncations and mutations introduced into the CTD of Rpf2. Amino acids 301–344 are represented by dashed lines. b Growth of yeast strain containing the genomic allele of rpf2Δ255-344 and its wild-type control. Serial dilutions (1 : 10 to 1 : 10,000) were spotted onto solid medium and incubated at 16, 25, 30, and 37 °C. Source data are provided as a Source Data file.