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. 2020 Jul 27;11:3751. doi: 10.1038/s41467-020-17534-5

Fig. 4. Changes in H68-70 are not caused by the absence of Sda1, which binds to H68-69.

Fig. 4

a Maturation of H68-H71 in wild-type cells during the transition from Nog2 particles to Rix1/Rea1 particles, then Nmd3 particles, and finally to mature 60S subunits (PDB IDs 3JCT [https://www.wwpdb.org/pdb?id=pdb_00003jct], 5FL8 [10.2210/pdb5FL8/pdb], 5APN [10.2210/pdb5APN/pdb], and 4V88 [10.2210/pdb4v88/pdb]). b Left: partial structure of Sda1 from the Rix1/Rea1 (Nog2 state 2) particle superimposed onto the structure of the Nog2 particle (PDB ID 3JCT [https://www.wwpdb.org/pdb?id=pdb_00003jct]). Sda1 is in close contact with H68 and H69 of the Nog2 particle. Right: Sda1 is in close contact with H68 and H69 in the Rix1/Rea1 particle (PBD ID 5FL8 [10.2210/pdb5FL8/pdb]), and the conformation of H69 is different from that in the Nog2 particle, presumably due to binding of Sda1. c Subunit interface view of density maps from the wild-type and E2 class of the Sda1-depleted particles. Densities for Rpf2, Rrs1 and H68-70 were colored using Color Zone in Chimera. No densities for H68-70 are missing in the E2 class compared with the wild-type Nog2 densities. d The atomic model for the wild-type particle was fitted into density maps of wild-type and E2 particles, respectively. Densities for Rpf2 and H68-70 are present in particles missing Sda1.