Fig. 7. The NTD of Rsa4 is invisible, and likely flexible in the C4 class of rpf2Δ255-344.

a The subunit interface view of density maps from the wild-type RPF2 and the C4 class of rpf2Δ255-344 mutant particles. Densities for Rsa4 were colored using Color Zone in Chimera. b Atomic models for Rsa4 from the wild-type and C4 class of the rpf2Δ255-344 mutant were fitted into density maps of the wild-type and the C4 class mutant. The first 34 amino acids of the NTD of the Rsa4 are flexible and invisible in the absence of the CTD of Rpf2. c Atomic models for Rsa4 and Nog2 from the wild-type and the C4 class are colored and labeled. For clarity, only relevant side chains are shown. Conformational changes in Nog2 may affect Rsa4, because Nog2 and Rsa4 are in close contact at two points (rectangles): at the NTD and the globular domain of Rsa4. Enlarged rectangles show contact points between Rsa4 and Nog2.