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. 2020 Jul 27;11:3751. doi: 10.1038/s41467-020-17534-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a The subunit interface view of density maps from wild-type particles, the C1 class of rpf2Δ255-344 mutant particles, and the E1 class of particles from Sda1-depleted yeast. The atomic model for wild-type particles was fitted into density maps of the wild-type and E1 class of mutant particles. The atomic model for the C4 class of mutant particles was fitted into the C1 class of mutant particles. b Enlarged view of the density maps from a. Densities for the ITS2 spacer rRNA and associated AFs Rlp7, Cic1, Nop7, Nop15, and Nop53 (all green), rRNA helices H75-79 (purple), and Bud20 (brown) are missing in the C1 and E1 classes. H68-70 (red) are missing in the C1 class, but are not significantly affected in the E1 class of mutant particles. (c) Enlarged portion of the density maps from (a). The L1 stalk (colored in pink) is in an unusual position in the C1 and E1 classes of particles, deflected toward H81.