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Cleavage of RNA substrates by APE1 in the presence of 5 mM MgCl2. (A) PAGE analysis of the reaction products. The positions of hydrolyzed nucleotides are indicated by arrows. (B) Comparison of cleavage efficiencies of RNA substrates by APE1, [APE1] = 2 μM, [RNA] = 1 μM, T = 25°C, reaction time = 1 h
