Table 1.
RNA substrate, designation | Nucleotide sequence |
---|---|
HP1 | 5′ FAM-r(AUAUAAGAUUAUAU)-BHQ1 3′ |
HP2 | 5′ FAM-r(AUAUAAGAAUUAUAU)-BHQ1 3′ |
HP3 | 5′ FAM-r(AUAUAAGAUAUUAUAU)-BHQ1 3′ |
HP4 | 5′ FAM-r(AUAUAAGAUCAUUAUAU)-BHQ1 3′ |
HP5 | 5′ FAM-r(AUACAACAUAAUUGUAU)-BHQ1 3′ |
HP6 | 5′ FAM-r(AUAUAACAUCAUUAUAU)-BHQ1 3′ |
L | 5′ FAM-r(AGAGAGGCAGAGA) 3′ |
Note.
FAM – 6-carboxyfluorescein label; BHQ1 –black hole quencher.
BHQ1 – black hole quencher.