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The values of the dissociation constant Kd
| Substrate | Buffer | Kd, μM | |
|---|---|---|---|
| Fluorescence titration | MST | ||
| HP1 | EDTA | 13.5 ± 8.2 | 12.1 ± 4.3 |
| HP2 | –«– | 7.8 ± 5.5 | 4.6 ± 0.9 |
| HP3 | –«– | 4.9 ± 1.1 | 2.6 ± 0.6 |
| HP4 | –«– | 3.0 ± 0.7 | 3.6 ± 1.3 |
| HP5 | –«– | 3.1 ± 1.0 | 2.5 ± 0.6 |
| HP6 | –«– | 2.2 ± 0.5 | 2.7 ± 0.5 |
| Mg2+ | 2.8 ± 0.6 | – | |
| Ca2+ | 1.6 ± 0.3 | – | |
| Mn2+ | 3.6 ± 0.8 | – | |
| L | EDTA | 1.5 ± 0.3 | – |
