Fig. 1.

The effect of mambalgin-2 on the acid-sensing sodium channels in the K562 cells. A – Analysis of the mRNA expression of various ASIC types using real-time PCR (n = 3). B – Activation of the channels caused by rapid changes in the extracellular solution pH from 7.4 to 5.0; membrane voltage was clamped at –50 mV. C – Currents activated by pH 5.0, before, during, and after 1 μM benzamil addition to the extracellular solution. D – Currents activated by pH 5.0, before, during, and after 1 μM mambalgin-2 addition to the extracellular solution