Fig. 3.

The effect of mambalgin-2 on the cell cycle in the K562 cells. A – Representative histogtamms of cell nuclei population distribution after 72 h incubation in absence (control) or presence of mambalgin-2. B – % of cells in each cell cycle phase. The data are presented as % of cells in each cell cycle phase ± SEM (n = 4); ** (p < 0.01) and *** (p < 0.001) indicate the significant difference between the control (untreated cells) and mambalgin-2-treated cells according to the two-tailed t-test. C – Representative Western blot showing the influence of mambalgin-2 on the phosphorylation of the cell cycle regulators. D – Quantification of the band intensities of cell cycle regulators after the cells were incubated with mambalgin-2. Data are presented as normalized to the β-actin band intensity, where untreated cells are taken as the control (100%, dashed line) ± SEM (n = 4); * (p < 0.05) indicates the difference between the control (untreated cells) and the cells treated with mambalgin-2 according to the two-tailed t-test