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Primers used for real-time PCR
| Gene | Primer | Amplicon, bp | |
|---|---|---|---|
| Forward | Reverse | ||
| β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | 88 |
| GPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC | 73 |
| RPL13a | TCAAAGCCTTCGCTAGTCTCC | GGCTCTTTTTGCCCGTATGC | 104 |
| ASIC1a | CGAAGCAGGCATCAAAGTGC | TTTGGATGATAGGGAGCCACG | 642 |
| ASIC2 | CACCAAGACTTCACCACAGTGTTT | TGTAGCGGGTCTCACAGTCA | 409 |
| ASIC3 | TACAAGAACTGTGCCCACCC | GGTCTTCGGAACAGAGCAGA | 502 |
| ASIC4 | GAGGAGAGAGACAAGCGGCA | GTCCAGCATGATCTCCAGGC | 930 |
