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. 2020 Jul 27;11:3744. doi: 10.1038/s41467-020-17560-3

Fig. 1. NLG2 KO mice exhibit spontaneous SWDs and behavior arrests.

Fig. 1

a–d Representative EEG and EMG traces showing SWDs in NLG2 KO (a), but not in WT (b), NLG1 KO (c), and NLG3 KO mice (d). Scale bars: 2 s/100 μV. e Averaged EEG recordings (3 h light period and 3 h dark period) showing increased SWD proportion (p < 0.0001), number (p < 0.0001), and duration (p < 0.0001) in NLG2 KO (n = 10) compared to WT mice (n = 8). Two-sided t-test. f Averaged EEG recordings (3 h light period and 3 h dark period) showing predominant presence of SWDs in the wake and REM sleep in NLG2 KO mice (proportion of time: p < 0.0001, n = 6; duration: p < 0.0001, n = 6; one-way ANOVA). g Relative power of various EEG frequencies showing significant genotype-frequency interactions (repeated two-way ANOVA) for the wake (p = 0.0001, WT: n = 6, NLG2 KO: n = 8, post-hoc Holm–Sidak’s comparisons: p < 0.0001 for the 5–8 Hz bin, p = 0.0004 for the 9–12 Hz bin) and REM sleep (p < 0.0001, WT: n = 6, NLG2 KO: n = 7, post-hoc Holm–Sidak’s comparisons: p < 0.0001 for the 5–8 Hz bin, p < 0.0001 for the 9–12 Hz bin), but not for the NREM sleep (p = 0.7788, WT: n = 6, NLG2 KO: n = 8). h Relative power of theta activity (5–8 Hz bin) over the 24 h EEG recordings showing significant genotype effect (repeated two-way ANOVA) for the wake (p < 0.0001, WT: n = 6, NLG2 KO: n = 7, post-hoc Holm–Sidak’s comparisons: significant at each time point) and REM sleep (p = 0.0003, WT: n = 5, NLG2 KO: n = 6, post-hoc Holm–Sidak’s comparisons: significant at each time point), but not for the NREM sleep (p = 0.0897, WT: n = 5, NLG2 KO: n = 7). Data in e–h represent mean ± SEM. **p < 0.01, ***p < 0.001. ns nonsignificant. Source data are provided as a Source Data file.