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. 2020 Jul 27;11:3744. doi: 10.1038/s41467-020-17560-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Injection sites of AAV-NLG2-GFP viruses in the ventrobasal thalamus. b Expression of AAV-NLG2-GFP. Scale bar: 1 mm. Images are representatives of five replicates. c Expression of NLG2-GFP (green), Neurotrace (blue) and GFAP (red) in cortical sections infected with AAV-NLG2-GFP viruses. Scale bars: 50 μm. Images are representatives of three replicates. d Summary graph of cortical staining showing NLG2-GFP colocalized with Neurotrace but not GFAP (p < 0.0001, n = 8). e Expression of NLG2 (red) in NLG2 KO mice injected with CAG-NLG2-GFP, but not in NLG2 KO mice injected with CAG-GFP control viruses. Scale bars: 20 μm. Images are representatives of six replicates. f High-magnification images showing expression of NLG2 (red, arrows) on thalamic neuronal surface (green) in NLG2 KO mice injected with CAG-NLG2-GFP viruses. Scale bars: 10 μm. Images are representatives of six replicates. g Sample traces of sIPSCs in NLG2 KO and WT mice injected with CAG-NLG2-GFP or CAG-GFP viruses. Scale bar: 1 s/20 pA. h Increased sIPSC amplitude (p = 0.0009) and frequency (p = 0.0437) in NLG2 KO thalamic neurons expressing CAG-NLG2-GFP (n = 15) compared to CAG-GFP (n = 10). Similar sIPSC amplitude (p = 0.2363) and frequency (p = 0.8983) between WT (n = 25) and NLG2 KO (n = 15) thalamic neurons expressing CAG-NLG2-GFP. i Decreased SWDs (p = 0.0019) in NLG2 KO mice virally expressing CAG-NLG2-GFP (n = 6) compared to CAG-GFP control (n = 7), and a similar reduction (p = 0.3858) in SWDs with viral expression of CAG-NLG2-GFP (n = 6) compared with CamKII-NLG2-GFP (n = 7). Scale bar: 2 s/100 μV. j Increased travel distance (p = 0.006), speed (p = 0.0058), mobile time (p = 0.0048), and decreased immobile time (p = 0.0048) in NLG2 KO mice injected with CAG-NLG2-GFP (n = 9) compared to control CAG-GFP viruses (n = 7). k Increased amplitude (p = 0.0153) and frequency (p = 0.0185) in NLG2 KO thalamic neurons virally expressing CamKII-NLG2-GFP (n = 9) compared to CamKII-GFP control (n = 8). Scale bar: 1 s/20 pA. l Increased travel distance (p = 0.0265), speed (p = 0.0235), mobile time (p = 0.0315), and decreased immobile time (p = 0.0316) in NLG2 KO mice injected with CamKII-NLG2-GFP (n = 9) compared to control CamKII-GFP viruses (n = 9). Data in d, h–l represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 with two-sided paired t-test. ns nonsignificant. Source data are provided as a Source Data file.