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. 2020 Jul 27;11(7):589. doi: 10.1038/s41419-020-02787-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Diagram of control group and RFA group. RFA treatment was performed on the left side tumors (RFA side). The right side (non-RFA side) tumors were used to further analysis. b H&E staining of tumor tissues on RFA and non-RFA side after RFA treatment. Scale bar = 50 μm (red line at the bottom right). c After 2 weeks, tumors from control group and RFA group were harvested and measured. Tumor volumes were compared to each two groups. p values were calculated based on a Student’s t test (n = 4 per group). d, e Percentage of CD8⁺PD-1⁺ cells in distant non-RFA tumor on day 3, 5, and 8 after RFA treatment. p values were calculated based on a Student’s t test (n = 3 per group).