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. 2020 Jul 27;11(7):589. doi: 10.1038/s41419-020-02787-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — RFA Treatments were initiated on the left side in RFA group when the tumor volume reached ~500 mm³, whereas the tumor on the right side did not receive any intervention. Tumors were harvested on day 3 after RFA treatment, and then digested and sorted for CD45⁺ immune cells. CD45⁺ immune cells were pooled together from three mice in each group and then subjected to scRNA-seq and TCR sequencing using a 10× Genomics pipeline.