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. 2020 Jul 27;3:396. doi: 10.1038/s42003-020-01134-6

Fig. 4. FhMYB27 and FhMYBx affected MBW complex forming by interacting with bHLH regulators detected in protoplasts.

Fig. 4

a Protein–protein interactions between FhMYB27 or FhMYBx and the MBW components detected by Gal4: GUS system in Arabidopsis protoplasts. FhMYB27ΔC indicated the modified version of FhMYB27 whose C-terminus was removed. GUS activities were detected after protoplasts incubation for 21 h. Data represented the mean ± SD of three biological replicates. Student’s t-test was used to analyze the significant difference (* p < 0.05; ** p < 0.01). b BiFC analysis of the interactions between FhMYB27 or FhMYBx and the MBW components in Freesia protoplasts. The fluorescence was observed under fluorescence microscope after incubation for 21 h. Bars indicate 25 µm.