Figure 3.

Malodour producing staphylococci PatB enzymes are selective for Cys-3M3SH. Comparison of catalytic efficiencies (K_cat/K_M) for selected PLP dependent C-S lyases against classical C-S lyase substrate l-cystathionine (y-axis) and l-Cys-3M3SH (x-axis). We show distinct clusters of activity across the PLP-DEs. MetC enzymes are selective for cystathionine only while G1 malodour-producing staphylococci are substrate selective for Cys-3M3SH, we now classify these enzymes as cysteine-thiol lyases. Other PatB orthologs (blue) display activity across both substrates whereas orthologous PatB enzymes from staphylococci (G2 & G3) show little or no activity with either substrates. Groups of staphylococcal PatB enzymes are highlighted.