Figure 1.

Preferential generation of memory Th1 and Th17 cells from effector CD30^hi Th cells. (A,B) in vitro-differentiated DO11.10 Tg CD30^hi, or CD30^lo Th1 (A) or Th17 (B) cells (5 × 10⁶) were transferred into BALB/c mice, and the mice were analyzed 1 month later (the protocol shown in Supplemental Figure 1F). Representative CD4/KJ1.26 profiles of the transferred cells in the lung, liver, spleen, and mesenteric lymph node (MLN) (upper panels) and the absolute cell numbers of CD4⁺KJ1.26⁺ transferred cells in three individual animals are shown (lower panels). Data are representative of three independent experiments. (C) Cytokine profiles of splenic CD4⁺KJ1.26⁺ memory Th1 and Th17 cells recovered from the host mice as in (A,B) are shown. Whole splenocytes were restimulated with PMA + Ionomycin for 4 h in the presence of monensin, and performed intracellular staining to detect the indicated cytokines. (D,E) in vitro-differentiated Th1 (D) or Th17 (E) cells (3 × 10⁷) from OTII Tg Cd30^+/+ or Cd30^−/− mice (Ly5.2) were transferred into Ly5.1 mice and analyzed 1 month later (the protocol shown in Supplemental Figure 1F). Representative CD4/Ly5.2 profiles of the transferred cells in the indicated tissues (upper panels) and the absolute cell numbers of CD4⁺Ly5.2⁺ transferred cells are shown (lower panels). Data are representative of at least two independent experiments. Mean values with SDs (n = 3) are shown (**P < 0.01, *P < 0.05).