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. 2020 Jul 27;11(7):586. doi: 10.1038/s41419-020-02768-4

Fig. 5. Upregulation of HIF-1α promotes IM resistance of GIST cells via binding to the promoter region of PGD gene.

Fig. 5

a Levels of HIF-1α protein in GIST cells. b Levels of HIF-1α protein in GIST tissues. c Levels of ferritin, PHD2, p-p65 and p65 protein in GIST cells. d Schematic representation of the luciferase plasmid with BS1, BS2, and BS3 in promoter region of PGD gene (left) and a luciferase reporter assay was used to evaluate interactions between HIF-1α and the targeting sites of PGD gene (right). e Schematic diagram exhibiting three potential HIF-1α binding sites in promoter region of PGD gene. f ChIP analysis was performed in GIST-T1R cells. A relatively brighter band was detected in HIF-1α compared with the control IgG with BS1. No band was observed with BS2 and BS3. g HIF-1α expression levels were evaluated by WB after HIF-1α knockdown in GIST-T1R cells. h Levels of PGD protein in GIST-T1 cells with HIF-1α knockdown. i Resistant capacity was evaluated by IC50 after HIF-1α knockdown in GIST cells. j FACS analysis (above) and statistical results (below) of ROS levels of GIST-T1 cells with HIF-1α knockdown. The error bars represent the mean (n = 3) ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001.