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. 2020 Jul 27;10:12497. doi: 10.1038/s41598-020-69236-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of SIRT7 silencing on barrier permeability in endothelial cell cultures. (A) Effect of SIRT7 silencing in HPAEC on endothelial barrier permeability measured by XPerT assays 6 h after stimulation with LPS 50 µg/ml or no stimulus. Average fluorescence values and standard deviations of 6 images expressed as fold changes relative to the average value of unstimulated, CTRL-silenced cultures are shown below the image. (B) Representative IF images for F-actin stress fibers in CTRL- or SIRT7-silenced HPAEC 48 h after transfection. Average pixel intensities and standard deviations of 6 images per condition are shown. (C) Phosphorylated and total β-Catenin protein levels 6 h after LPS stimulation or no treatment in CTRL-silenced (□) or SIRT7-silenced (■) HPAEC cultures. Densitometry measurements of phosphorylated β-Catenin relative to total β-Catenin or α-tubulin (also shown in Supplementary Figure 11E) and total β-Catenin relative to α-tubulin (shown in Supplementary Figure 11C) are shown. Each bar represents the average of two separate samples per condition. Significant differences (P < 0.05) are denoted by stars. P values were calculated using the Student’s t test. For (C), different proteins for the same group of samples are demarcated by white spaces.