Fig. 9. The expression profiles of 13 selected FvSTs obtained from quantitative real-time PCR analysis across 19 strawberry varieties.

The amount of the cDNA template in each sample was normalized by the amplification of FaCHP1 and FaGAPDH1⁶⁸. FvST expression was hierarchically clustered into a heatmap using the HemI package⁶⁹. The color scale represents log2-transformed relative mRNA levels, where red indicates a high level, blue a low level, and white for an intermediate level. Distinct varieties (A-S) are arranged in the same order as in Fig. 8b. Two independent biological repeats and three technical replicates were performed for the measurements of fruits of each genotype at three stages (green, white, and turning)