Figure 2.

Intracellular localization of CrPHOT is similar to that of FPK1p regardless of BL irradiation. (a) Punctate structures of GFP-CrPHOT and GFP-KDm stained with FM4-64 were similar to that of GFP-FPK1p. Yeast fpk1Δ fpk2Δ cells carrying pRS416-GFP-FPK1, -GFP-CrPHOT or -GFP-KDm were incubated at 25 °C with FM4-64 under each light condition, followed by confocal microscopic observation. (b) Confocal images of KKT332 (fpk1Δ fpk2Δ DNF1-GFP) cells carrying pKT1639 (pRS416-mRFP-FPK1), pRS416-mRFP-CrPHOT or -mRFP-KDm. In (a) and (b), yeast cells were grown to logarithmic phase in darkness (Dark) or under 10 μmol m⁻² s⁻¹ BL irradiation (Blue) at 18 °C. Images were merged to compare the two signal patterns. Scale bars = 5 μm.