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. 2020 Jul 27;10:12474. doi: 10.1038/s41598-020-69459-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optical control of actin depolarization associated with apical-isotropic growth switching. (a, b) Optical control of F-actin distribution by CrPHOT. Yeast fpk1Δ fpk2Δ cells carrying pKT1639 (pRS416-FPK1) or pRS416-CrPHOT were cultured in darkness (Dark) or under 10 μmol m^-2 s^-1 BL irradiation (Blue) at 18 °C, followed by staining with phalloidin-TRITC and DAPI to visualize actin and nuclei, respectively. (a) A representative cell image by microscopic observation. Scale bar = 5 μm. (b) Large-budded cells with divided nuclei were classified as showing actin polarized to the bud tip (grey) or its distribution in whole daughter cells (black). n = 155–253. (c, d) Optical control of Myo2p-GFP localization in a kinase-dependent manner. KKT353 (fpk1Δ fpk2Δ MYO2-GFP) cells carrying pKT1639 (pRS416-FPK1) or pRS416-CrPHOT or its derivatives were cultured in YPDA medium in darkness (Dark) or under BL irradiation (Blue) at 18 °C, followed by staining with DAPI to visualize nuclei. (c) A representative cell image obtained by microscopic observation. Arrowheads indicate Myo2p-GFP polarized to the bud tip. Scale bar = 5 μm. (d) Large-budded cells with divided nuclei were classified as showing Myo2p-GFP distributed in whole daughter cells (black), polarized to the bud tip (grey) or delocalized (white). n = 96–180.