Figure 10.

IGF-1 reduces airway inflammation in ALI mice. (A–D) ALI model mice were prepared as described in Materials and Methods (Antibodies and Reagents). At 24 h before LPS nasal drops, BALB/c mice received nasal drops of IGF-1 (0.8 mg/kg) once every 12 h. At 24 h after LPS treatment, lung tissues and BALF were collected from mice. (A) The lung tissues of each group were stained with HE, and the pathological damage was observed under a microscope. (B) The BALF of each group of mice was centrifuged, cell pellets were smeared and stained with Wright's staining solution, and the number of neutrophils was counted. (C) Wet-dry weight ratios of lung tissues in each group of mice were calculated as described in Materials and Methods (Western Blot Analysis). (D) The total protein content of BALF in each group of mice was measured according to Materials and Methods (Wet-Dry Weight Ratio of Lung Tissue). **P < 0.01 vs. the control group, ^##P < 0.01 vs. the ALI group.